European Journal of Cardiovascular Medicine

Introduction: Treatment of bacterial infections has become complicated due to the emergence of multidrug resistant strains of gram negative bacilli. The multidrug resistant strains of gram negative bacilli causes multiple clinical infections and has become a rising problem globally. The metallo beta lactamases encoding genes are very sever in gram negative bacteria such as E.coli. Metallo beta lactamases are beta lactamase enzymes produced by pathogenic bacteria and gradually found in gram negative organisms Materials And Methods: This is a prospective, descriptive, Cross sectional, In-vitro laboratory based , single center study in the Department of Microbiology, Index Medical College, Hospital and Research center Indore. The isolates will be obtained from clinical specimens such as blood, urine, exudative specimens which included pus, wound swabs, eye swab, ear swabs, conjunctival swabs, cerebrospinal fluid, pleural fluid, peritoneal fluid, drain fluids and cerebrospinal fluid (CSF) and lower respiratory secretions (bronchial wash, endotracheal aspirates and sputum) and other relevant clinical material submitted to Microbiology Laboratory for Culture and Sensitivity testing.  Result: Among 210 isolates, 81 isolates were Acinetobacter baumanni, 30 isolates were Acinetobacter lwofii, 23 isolates were of other Acinetobacter sp. and 76 isolates were Pseudomonas aeruginosa. Among 210 isolates, majority were collected from male patient (160). In case of Acinetobacterbaumanni isolates, 50 were collected from male patients whereas 26 isolates were collected from female patients.In case of Acinetobacter lwoffi, 27 isolates were from male and only 03 isolates were from female. AmongAcinetobacter sp.08 isolates were from male patients and only 05 were from female patient. Similarly in case of Pseudomonas aeruginosa isolates, 65 were from male and 16 isolates from female patient. Conclusion: All the MBL positive isolates showed resistance towards aminoglycosides. In case of Pseudomonas aeruginosa Colistin was 100% sensitive and the next effective drug was Amikacin.In Acinetobacter the most effective drug was Tigecyclin(100% sensitivity) and the next effective drug was found to be Tobramycin and Gentamycin. In most of the MBL positive isolates Aztreonam was resistant. This can be attributed to the co presence of multiple mechanism of resistance in MBL positive isolates.