European Journal of Cardiovascular Medicine

Unalike parts of Crocus sativus plant have revealed important biological activities. In folklore petals and roots of crocus sativus plant, are used to treat various diseases like diarrhea, Gastritis, cold, bronchitis, cough, Diabetes, and Infections. The present study was designed to explore in vitro anti-inflammatory potential of Crocus Sativus stigmas. Crocus Sativus has been used conventionally for different inflammatory disorders. A few results about in vitro anti-inflammatory activity of extract fractions have been published until now. Hence, aim of this study was to evaluate in vitro anti-inflammatory potential of Crocus Sativus ethanolic extract methanolic and petroleum benzine fraction. Serial dissolutions were prepared at 125, 250 and 500μg/mL from each extract fraction of crocus sativus stigmas, which were used to evaluate anti-inflammatory activity in vitro by protein denaturation method, using Aspirin as standard drug. Both extract fractions were able to exhibit major anti-inflammatory activity. However, methanolic extract fraction revealed the highest percentage inhibition, suggesting major concentration of compounds with promising anti-inflammatory effect. So with these findings results can be concluded that crocus sativus stigmas possess in vitro anti-inflammatory properties at different concentrations Percent inhibition of protein denaturation of these extract fractions was  within the range of 33.0–59.25%, for petroleum benzine fraction and for methanolic fraction  46.94 to 63.74 and  exhibited a significantly higher (p < 0.001) inhibition level.