Background: Mupirocin is a powerful topical antibiotic for treating carriers of MRSA. Resistance to this medication has grown as a result of its frequent use in clinical settings and over-the-counter accessibility. Concern is also raised by Staphylococcus aureus having Mupirocin resistance. Materials and Methods: It is a cross sectional study conducted in in Department of Microbiology during the period of May 2016 to April 2017. 1200 patients were selected in the study. Out of which, 400 patients were inpatients, 400 patients from community and 400 were from health care workers