Abstract Introduction: Throughout ancient times, tuberculosis has posed a serious threat to humankind. Around 150 million years ago is when the Mycobacterium genus was first recognised. Egyptian mummies have been used to show the skeletal malformations caused by TB. Yet, the infectious agent behind this terrible sickness was not identified for quite some time. Material and Methods: Between January 2022 and October 2022, the epartment of Microbiology at the Ayaan Institute of Medical Sciences in Moinabad, Telangana conducted a prospective study. A total of one hundred people were included as part of this study's sample. Results: Children who had expressed concerns were asked for and given permission to have a sample taken by their parents or legal guardians. Gastric aspirates, broncho-alveolar lavage, induced sputum, sputum, tracheal aspirate, ascitic fluid, lymph node aspirates, pleural fluid, and a synovial biopsy were all included in the collection of specimens. There was complete processing and analysis of all samples. Conclusion: This research was conducted to identify the most efficacious techniques for detecting TB in young people. CBNAAT has an advantage over other methods used to diagnose paediatric TB because it picked up more instances that were overlooked by those other approaches