Background and Aims: One of the most common illnesses with high morbidity and death is urinary tract infections (UTIs). Even though a broad variety of medicines are available for the care of UTI, uropathogens have developed resistance, leading to a major treatment failure. In order to assess the rate of pathogen isolation as well as the pattern of antibiotic susceptibility of microorganisms causing UTI, the current investigation was conducted. Materials and Methods: 350 urine samples from individuals who had a UTI that was clinically suspected were processed between January and March 2019. The susceptibility of various antibiotics and UTI isolates were studied using the Kirby-Bauer disc diffusion method. Results: In this study, among the 350 samples, 195 (55.7%) samples showed microbial growth. Out of 195 isolates, 33 were Candida spp., and 162 were bacteria. E. coli (46.29%) was the predominant isolate, followed by E. faecalis (23.45%) as well as Klebsiella spp. (19.75%). E. coli was most susceptible to nitrofurantoin (96.29%), and Candida spp was most susceptible to Fluconazole (90.90%). Conclusion: The study outcome showed that E. coli is the major uropathogen responsible for UTI, and Nitrofurantoin is the superior antibiotic with a high susceptibly rate