European Journal of Cardiovascular Medicine

Introduction: In the beginning of year 2022, India facing the third wave of Covid19 experiencing the all time high number of Covid19 cases throughout the nation. This is need of the hour to know who are more prone to this latest variant of SARS CoV2 - Omicron (B.1.1.529). Aim and Objective: The present study was aimed to analyze the association of age, sex and ABO blood group with the seroprevalence of anti-SARS-CoV-2 IgG antibodies among healthy blood donors during the 3rdwave of the COVID-19 pandemic in India, to monitor and help in planning restrictive and preventive measures for COVID-19 infection. Material and Method:This was a blood centrebased, cross-sectional,sero-prevalence study conducted on blood donorsduring the 3rd wave of the Covid-19 pandemic (Jan-April 2022) under the department of IHTM at Government Medical College, Kota (Rajasthan) India; after getting the permission from the Departmental Research Committee and Institutional Ethical Committee.All participants were healthy blood donors who donated blood atthe blood centres underGovt. Medical College and Attached Group of Hospitals, Kota, during Jan 2022 to April 2022. Samples were tested for the SARS-CoV-2 IgG antibody by using CE-markedErbaLisa COVID-19 IgG Antibody Detection ELISAkits based on the principle of indirect ELISA using recombinant spike subunit antigen, developed by Erba Mannheim and Calbiotech. Optical density was measured by calorimetric reaction at a reading wavelength of 450nm to detect the reaction between SARS-COVID-19 IgG Antibodies and Antigens.The statistical analysis was done using IBM SPSS software version 22.0.P-value<0.05 was considered statistically significant. Results:In the present study,1320blood donors were enrolled andscreened. Out of them, 97.6% (1288/1320) were found sero-reactive and remaining 2.4% (32/1320) were nonreactivefor SARS-CoV-2 IgG antibody. Most of them were male donors 1280(97.0%) and female donors were very less 40(3.0%).No statistically significant correlation could be established between age, sex and blood group of the blood donors and sero-reactivity(P=1.000; 0.624 and 0.938 respectively). Conclusion:Present study concludes that majority of the population has beenalready exposed to SARS-CoV-2 infectionand widespread coverageof covid-19 vaccination and immunity (SARS-CoV-2 IgG antibodies) develops afterCovid19 infections and its vaccination, irrespective of persons’ age, sex and blood type (ABO RhD).