European Journal of Cardiovascular Medicine

Introduction: Methicillin–resistant staphylococcus aureus are among the most common cause of microbial   infections in humans. It encompasses a wide array of infections, accounting for a vast number of community as well as hospital acquired infections in developing countries. Thus, it is of utmost importance to distinguish between hospitals acquired infection and community infection of MRSA which that hospital acquired in complication of MRSA infecting pathogens are more likely to be resistant to antimicrobial agents and lead to higher mortality. Recently, mec C gene has also been found to be associated with MRSA. It code for PBP2c in contrast to PBP2a of mecA gene. Hospital personnel harboring MRSA have been implicated as the chief source of nosocomial infection. Aim: To identify the MRSA isolates from clinical specimens to determine the antibiotic resistant pattern of the MRSA isolate and identify the mec A gene in all resistant pattern strains. Materials and Methods:  It’s cross sectional study from March July 2020 to February 2021 where 150 isolate samples of methicillin resistance staphylococcus aureus (MRSA) isolated from various clinical samples were included in the study in of microbiology department, index medical college Indore M.P. Phenotypic cultures and antimicrobial resistant test was performed from isolated pathogens using Kirby- Bauer disk diffusion method and correlation with genotypic mecA isolates by RTPCR. Results: In our study significant MRSA bacteriuria accounted for 150 (46.6%) were isolated samples.In this study of maximum numbers of isolates were from MRSA in the age group of 30-40 yrs 25 (28.7%) followed by 20 (22.9%) between age group of 50-60 yrs. Among gram positive Cocci in Methicillin resistant staphylococcus aureus was 87 (58%) followed by MSSA 62 (42%). Isolated pathogens were sensitive to linezolid (100%), vancomycin (100%) followed by cotrimoxazole (31%), amikacin (48.3%) and showed higher resistance to penicillin (100%) followed by cefoxitin 87 (100%) in MRSA. This present study MRSA 100% specific were results of mecA gene detection by RTPCR. Conclusion: In this study, male were mostly affected then female and the most common organisms was staphylococcus aureus in gram positive cocci bacteria and sensitive to linezolid, vancomycin and resistance predominantly to Cefoxitin and penicillin. The sensitivity and resistance pattern of to common antimicrobial agents must be taken into account when selecting treatment plans for MRSA. Among RTPCR for mecA genes remains to be rapid molecular method for the evaluation of MRSA. In this study, mecA has been detected in all of the MRSA isolates were 100% sensitivity then phenotypic method, where Cefoxitin of MIC detection by E-strip method were 98.5 sensitivity followed by  cefoxitin disc diffusion method were 97.5 sensitivity.