Introduction: Breast abscess is a painful condition that usually appears as a lump and is brought on by infection. Since it's one of the most prevalent medical conditions affecting women, it's critical to understand the most recent developments in isolated bacteria and antibiotics that can be applied empirically. The aim of present study is to evaluate the role of different antibiotics in breast abscess, efficacy of empirical antibiotic therapy and to find out the microbiology of the disease. Material and methods: The present ambispective study was conducted at E.S.I.C. Medical College and Hospital among females presented with breast abscess for a period of 2 years. Total cases selected were 160 based on inclusion and exclusion criteria. Statistical analysis was done using SPSS version 25.0. Level of significance was kept at p<0.05. Results: Maximum were in age group of <30 years (47.5%). Most common symptoms were pain (100%) and redness (85%). Examining subjects revealed that maximum 85% of subjects had Mastitis. The most common mycobacteria in abscess was MRSA (30%), followed by E.coli (15%). Linzolid was mostly susceptible with MRSA, Amikacin with E.coli and S.typhi, Gentamycinmycin/tetra with S.aureus. Conclusion: The majority of breast abscess cases involved the isolation of methillin-resistant Staphylococcus aureus. When choosing an empirical treatment for a breast abscess, understanding the microbiological profile and antibiogram of the bacteria is crucial.