European Journal of Cardiovascular Medicine

Introduction: Opportunistic infections of gram negative non fermenters cause significant increase in morbidity and mortality of critically ill patients. Materials And Methods: All the samples were collected by following aseptic precautions, among which 200 pathogenic isolates were obtained which formed the study group. All the samples were processed for microscopic examination, culture and sensitivity testing according to CLSI protocols. Results:Out of 200 clinical isolates, 42 different non-fermenters were studied .Among these the predominant non-fermenter was Pseudomonas. aeruginosa 30(71.4%), followed by Acinetobacter baumannii 8(19%), Alcaligenes faecalis 2(4.7%) and Acinetobacter lwoffii 2(4.7%). Modified hodge test detected 6(66%) of Carbapenemase production by imipenem resistant isolates .E-Test detected more number of positive isolates that produced MBLs i.e.,5(55.5%), followed by Combined disk potentiation test 4(44%), and double disk synergy test 3(33%). Conclusion: The future of prevention in ICUs will likely be based on our ability to adapt policies and emerging technologies to specific risk profiles.