Background: Circadian rhythm is a biological and behavioral process that regulates sleep. The quantity and quality of our sleep significantly impact our day-to-day life and various health outcomes that help the body in cellular repair and fight diseases. In the modern era, sleep has become another casualty of modern life thereby making it very essential to assess and keep track of sleep quality. Sleep quality is assessed commonly using the Epworth Sleepiness Scale (ESS) and Pittsburgh Sleep Quality Index (PSQI). The comparison of concordance and discordance of ESS and PSQI is not done earlier and a thorough evaluation of their measurement results and degree of agreement is essential. Thus, the study aims to do a comparative analysis of ESS and PSQI scales and analyses their concordance in measuring sleep-related parameters. Methods: Healthcare employees working in the Parent Institute were included in this study. Subjects were given the Epworth Sleepiness Scale and the Pittsburgh Sleep Quality Index questionnaire to assess sleep quality and a comparative analysis of both scales was done. Results: The study compares both the scales – ESS (Adequate & inadequate sleep) & PSQI (Good & poor sleep) using Kappa statistics showing an overall agreement of 74.6% with a simple kappa value of 0.49 (Fair agreement) which was statistically significant (P < 0.001). Conclusion: The study shows significantly fair concordance between the ESS and PSQI in determining sleep parameters demonstrating that they each effectively assess different facets of sleep.