Aim and objective – present study was done to compare newly introduced gel card technique and conventional tube method. Methodology - Present study was retrospective study of one-year duration from April 2021 to June 2022 conducted in department of Pathology Govt Medical College Mahasamund (C.G). Total 2000 sample were taken. Blood grouping and Cross match of both recipient blood sample and donor blood sample were done using Both tube and gel technique and their results were compared and analyzed. Result – In our study 0.5% samples showed incompatibility with Gel card method but by test tube method 100% samples showed compatibility until subjected to IAT using AHG after incubation at 37°C for 60 minutes, where 0.5% samples incompatible with gel card method were also found to be incompatible. The sensitivity and specificity of both methods is 100% if use of AHG is included in all tests performed by tube method, otherwise the specificity of tube method is 99.5%. Conclusion – The gel technique has definite advantages over the conventional tube technique because of being able to detect more positive samples than tube and its shorter duration for the cross matching procedure.