Introduction: UTI is a prevalent condition that affects approximately 40% of the adult population. Clinical Syndrome may be characterized as Asymptomatic Bacteriuria, Uncomplicated Cystitis, Pyelonephritis, Prostatitis, or complicated UTI. The goals of treatment of urinary tract infection are to eliminate the pathogen, to prevent Urosepsis, and to reduce the risk of renal scarring. Objective: To study the culture and sensitivity patterns of urinary tract infections in asymptomatic patients in a tertiary care hospital. Methods: A total of 120 samples were collected from January to June 2023. Identification was conducted by conventional biochemical tests. Antibiotic sensitivity test was analysed by disc diffusion method using different antibiotics and their zone of inhibition was measured. Results: Out of 120 subjects enrolled, 67 were males and 53 females The most common urinary pathogen isolated was E. Coli (64%) sensitive most often (82% sensitivity) to Amikacin. Overall sensitivity of different urinary isolates to amikacin was highest (82%). Conclusion: Epidemiology and resistance patterns of bacterial pathogens in pediatrics urinary tract infection show large interregional variability, and rates of bacterial resistances are changing due to different antibiotic treatment. Knowledge of spectrum of pathogens and their patterns of drug resistance is indispensable for the empirical section of an effective therapeutic agent prior to availability of culture result.