Background: Despite recent advances in obstetrics, perinatal, and neonatal care, multiple pregnancies continue to be among the riskiest conditions for the mother, fetus, and neonate. Compared to singletons, twin pregnancies have higher rates of obstetric and perinatal complications. The objective of present study was to compare the neonatal outcome of the first twin with that of the second twin delivered by vaginal route. Methods: The present study was prospective observational study. A total of 90 women with twin pregnancies more than 28 weeks of gestation were included in the study. Study was conducted from July2021toJune2022 in the Department of Pediatric and Gynae& Obstetrics, Burdwan Medical College, Burdwan, West Bengal, India. Statistical data were analysed by using Microsoft Excel and SPSS V.20 software. Results: The age of the mothers ranged from 18-36 years with the mean age being 24.7±3,86 years. The incidence of twin deliveries was highest among 25-30 years Mean weight of 1st twin was 2.02 kg ± 0.420 kg and mean weight of 2nd twin was 2.0 kg ± 0.430 kg.Most women delivered twins vaginally, 72.22% (n=65) for first twin and 63.33% (n=57) for the second twin. Preterm labour was the most common complication constituting 71.11% followed by anaemia, hypertensive disorders. RDS is the most common neonatal morbidityconstituting 14.44%. for the first twin and 15.56% of the second twins which resulted in the need for ventilation.The mean 1 minute APGAR scorewas 7.46±1.67 for the first twin and 6.24±1.65 for the second twin. The comparison of 1 minute APGAR. score between first and the second twin was found to be statistically significant.Conclusion :It was observed in the study that the neonatal mortality and morbidity is encountered with preterm twin delivery, so its prevention, diagnosis and treatment are must.