European Journal of Cardiovascular Medicine

Background:-Tuberculosis poses a severe threat to human health. At present, compared with the traditional diagnostic methods for tuberculosis pleural effusion and sputum, such as sputum microscopy for Acid-fast bacilli (Z-N stain) and confirmatory molecular test for tuberculosis by Truenat MTB (PCR) was regarded as an emerging technology for its efficiency. We performed a systematic review and meta-analysis to evaluate diagnostic accuracy of a positive pleural effusion and sputum microscopy were better or useful tool for diagnosis and treatment of Tuberculosis. The study is implemented to check the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of sputum and pleural fluid were calculated, Material and methods- The study included 265 suspected pulmonary TB patients with positive clinical manifestations at Varun Arjun Medical College and Rohilkhand Hospital, Banthra, Shahjahanpur U.P. Sample was collected over age of 18 that had been suspected pulmonary tuberculosis with positive sign and symptoms from July 2023 to December 2023. The diagnostic results from pleural fluid and sputum sample were used as the standard calculating the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV). Data was collected from the Department of Microbiology, Varun Arjun Medical College and Rohilkhand Hospital, Banthra, Shahjahanpur U.P. Results: The sensitivity for smear microscopy is 85.2% (95% CI 77.8 – 92.1), specificity is 90.2% (95% CI 82.6 – 97.8), and positive predictive value is 83.7% (95% CI 82.6 – 84.9) and negative Predictive value is 69.4% (95% CI 63.6 – 74.4). In contrast, the sensitivity for truenat MTB is 92.5% (95% CI 91.1 – 93.9), specificity is 98.7% (95% CI 97.8 – 99.6), negative predictive value is 94.8% (95% CI 93.8 – 95.8) and positive predictive value is 97.1% (95% CI 95.7 – 98.5). Conclusion: This preliminary study shows that the Truenat MTB test allows detection of TB in Approximately in short time of interval and can be utilized in near- care setting to provide quick and accurate diagnosis