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Research Article | Volume 14 Issue:1 (Jan-Feb, 2024) | Pages 520 - 522
Evaluation of rapid immunochromatographic test for the early diagnosis of dengue virus infection and their comparative study by IgM capture Elisa in Tertiary Care Hospital, at PMCH, Patna
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1
Tutor (tenure), Department of Microbiology, Patna Medical College, Patna.
2
Assistant Professor, Department of Microbiology, Nalanda Medical College, Patna.
3
Assistant Professor, Department of Microbiology, Patna Medical College, Patna.
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Professor, Department of Microbiology, Nalanda Medical College, Patna
5
Professor and HOD, Department of Microbiology, Bettiah Medical College, Bettiah
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Professor and HOD, Department of Microbiology, Patna Medical College, Patna.
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Final year PG student, Department of Microbiology, Nalanda Medical College, Patna.
Under a Creative Commons license
Open Access
DOI : 10.5083/ejcm
Received
Dec. 4, 2023
Revised
Jan. 2, 2024
Accepted
Jan. 17, 2024
Published
Feb. 3, 2024
Abstract

Objective: The aim of present study was conducted to evaluate the rapid immunochromatographic tests that detect NS1antigen, IgM, IgG antibody simultaneously and their comparative study by IgM capture Elisa. Material and methods: A total of 368 clinically diagnosed patients of dengue fever were included in the study. From all the patients 368 blood samples were collected and serum were separated. From all the serum tests were performed by rapid immunochromatographic test kit which can detect all three parameter NS1Ag, IgM& IgG antibody according to packet insert by manufacturers guide lines. All the tests were compared by IgM capture Elisa. Result: Out of368 blood samples of dengue fever 136 samples were positive by rapid immunochromatographic test. Among 136 positive cases   127 cases were NS1Ag positive,05 cases are IgM reactive; 03 samples are IgG reactive and only 01 samples are reactive forNS1, IgM & IgG. Out of 368 blood samples tested by dengue Ig M capture Elisa only 123cases (33.42%) were reactive. Conclusion: Detection of dengue NS1Ag in the patient can be used as screening test in early cases of dengue illness. Since it is easy to perform it can be used in primary health care centres to take appropriate measure to reduce morbidity and mortality. Early notification of disease can awake the public health authority to take control measures.

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