Contents
Download PDF
pdf Download XML
218 Views
86 Downloads
Share this article
Research Article | Volume 6 Issue :2 (, 2016) | Pages 41 - 50
Recent Improvements in In Vitro Angiogenesis Assays
Under a Creative Commons license
Open Access
Published
June 12, 2016
Abstract
Angiogenesis research has grown extensively over the past few decades with the recognition that angiogenesis plays a key role in embryonic development, wound healing, and also pathological conditions such as tumor metastasis. Numerous in vitro angiogenesis assays have been designed to mimic in vivo angiogenesis process. The in vitro assays are quick, simple, quantitative, and cost-effective, and have thereby played valuable roles in evaluation of the effects of test compounds on angiogenesis. However, each in vitro assay represents an individual step of a complex angiogenic cascade. Furthermore, significant diversities exist within endothelial cell types and within basement membrane extracts used in the assays. Thus, choosing appropriate cell types and assay conditions that most closely resemble the disease-specific angiogenesis being studied is essential to obtain meaningful results. Performing multiple in vitro assays together should also be considered to get data that is translatable from the preclinical to the clinical stage. For these reasons, increasing our understanding of in vitro assays is necessary for proper assay performance as well as correct interpretation of the results. Here we discuss two main critical factors regarding endothelial cell types and basement membrane extracts that are known to largely affect in vitro angiogenesis assays. We also introduce recent improvements in the most widely-used in vitro angiogenesis assays, which are 2-dimensional (2D) tube formation assays and 3-dimensional (3D) spheroid angiogenesis assay.
Keywords
Recommended Articles
Research Article
Study Of Hematological Abnormalities in Decompensated Chronic Liver Disease
...
Published: 30/06/2025
Download PDF
Research Article
Role of Academic Research Consortium for High Bleeding Risk (ARC-HBR) criteria in prediction of clinical outcomes in patients with acute coronary syndrome
...
Published: 30/09/2025
Download PDF
Research Article
Comparison of Intravenous Magnesium Sulphate and Clonidine in Attenuating Hemodynamic Responses during Laryngoscopy and Tracheal Intubation: A Prospective Randomized Double-Blind Study
Published: 30/09/2020
Download PDF
Research Article
A Study Comparing Pre- Operative Prophylactic Iv Antibiotics with Combined IV and Intraincisional Antibiotics Administration for Reducing Surgical Site Infections
...
Published: 27/09/2025
Download PDF
Chat on WhatsApp
Copyright © EJCM Publisher. All Rights Reserved.