Contents
Download PDF
pdf Download XML
190 Views
86 Downloads
Share this article
Research Article | Volume 6 Issue :2 (, 2016) | Pages 41 - 50
Recent Improvements in In Vitro Angiogenesis Assays
Under a Creative Commons license
Open Access
Published
June 12, 2016
Abstract
Angiogenesis research has grown extensively over the past few decades with the recognition that angiogenesis plays a key role in embryonic development, wound healing, and also pathological conditions such as tumor metastasis. Numerous in vitro angiogenesis assays have been designed to mimic in vivo angiogenesis process. The in vitro assays are quick, simple, quantitative, and cost-effective, and have thereby played valuable roles in evaluation of the effects of test compounds on angiogenesis. However, each in vitro assay represents an individual step of a complex angiogenic cascade. Furthermore, significant diversities exist within endothelial cell types and within basement membrane extracts used in the assays. Thus, choosing appropriate cell types and assay conditions that most closely resemble the disease-specific angiogenesis being studied is essential to obtain meaningful results. Performing multiple in vitro assays together should also be considered to get data that is translatable from the preclinical to the clinical stage. For these reasons, increasing our understanding of in vitro assays is necessary for proper assay performance as well as correct interpretation of the results. Here we discuss two main critical factors regarding endothelial cell types and basement membrane extracts that are known to largely affect in vitro angiogenesis assays. We also introduce recent improvements in the most widely-used in vitro angiogenesis assays, which are 2-dimensional (2D) tube formation assays and 3-dimensional (3D) spheroid angiogenesis assay.
Keywords
Recommended Articles
Research Article
Diabetes and Cardiovascular Risk in Menopausal Women: An Emerging Concern
...
Published: 14/07/2025
Download PDF
Research Article
A Study of Serum Electrolytes and Uric Acid Among Psoriasis Patients in ACSR Government Hospital of SPSR Nellore District: A Case-Control Study
...
Published: 30/05/2025
Download PDF
Case Series
A rare case series on anticholinergic drug induced psychosis
...
Published: 29/04/2024
Download PDF
Research Article
A study on the incidence and pattern of transfusion reactions at a Tertiary Care Centre – Kurnool General Hospital
...
Published: 14/07/2025
Download PDF
Chat on WhatsApp
Copyright © EJCM Publisher. All Rights Reserved.