European Journal of Cardiovascular Medicine

Background: Urinary tract infection (UTI) is a common health problem in both community and nosocomial settings, affecting both men and women equally. Pseudomonas aeruginosa is an opportunistic human pathogen causing devastating acute and chronic infections in individuals with compromised immune systems. Biofilm is an architecture built mostly by autogenic extracellular polymeric substances which function as a scaffold to encase the bacteria together on surfaces, and to protect them from environmental stresses, impedes phagocytosis and thereby conferring the capacity for colonization and long-term persistence. So, the aim of this study to screen of some important quorum sensing and biofilm genes among pseudomonas aeruginosa isolated from UTIs patients. Methods: These study was conducted in Al-Qadisiyah province, Iraq at five major hospitals (AL-Diwanyia Teaching Hospital, Feminine and children teaching hospital, Afak General Hospital, AL-Hamzah General Hospital and AL-Shamiya General Hospital) during the period from (November, 2020 to June, 2021). A total of 800 urine samples were collected from male and females referring to five major hospitals. The age of the patients ranged from (1 to 80) years-old. Results: Sixty isolates were showed positive and identified as P. aeruginosa by using selective media, biochemical test system and VITEK-2 compact system. Genetically, in the present study, a total DNA was extracted from all clinical P. aeruginosa isolates. The current study revealed that all isolates showed difference in contain the ninth genes (lasR, rhlI, pelA, pslA, lecA, ndvB, tssc1, vfr and QscR), which was related with biofilm formation and Quorum sensing (QS) phenomenon. Conclusion: The study conclude the quorum sensing system play a important role in pathogenicity and biofilm formation of P. aeruginosa, and it’s an essential for bacteria to increase growth and resistant of antibiotics.